Biología reproductiva

1. Agarwal A, Said TM, Bedaiwy M, Nenerjee J, Alvarez JG . Oxidative stress in an assisted reproductive technique setting. Fertil Steril. 2006 Jul 21.

2. Gupta S, Agarwal A, Krajcir N, Alvarez JG . Role of oxidative stress in endometriosis. Reprod Biomed Online. 2006; 3:126-34.

3. Medrano A, Fernandez-Novell JM, Ramio L, Alvarez JG , Goldberg E, Monserrat Rivera JM, Guinovart JJ, Rigau T, Rodriguez-Gil JE. Utilization of citrate and lactate through a lactate dehydrogenase and ATP-regulated pathway in boar spermatozoa. Mol Reprod Dev. 2006; 73:369-78.

4. Alvarez JG . The predictive value of sperm chromatin structure assay. Hum Reprod, 2005; 20:2365 - 2367.

5. Alvarez JG . Efficient treatment of infertility due to sperm DNA damage by ICSI with testicular spermatozoa. Hum Reprod. 2005; 20:2031-2.

6. Alvarez JG , Larson-Cook KL and Evenson DP. Selecting cryopreserved semen for assisted reproductive techniques based on the level of sperm nuclear DNA fragmentation resulted in pregnancy. Fertil Steril, 2004; 81:712-713.

7. Freedman SD, Blanco PG, Zaman MM, Shea JC, Ollero M, Hopper IK, Weed DA, Gelrud A, Regan MM, Laposata M, Alvarez JG , O'Sullivan BP. Association of cystic fibrosis with abnormalities in fatty acid metabolism. N Engl J Med. 2004; 350:560-9.

8. Alvarez JG . DNA fragmentation in human spermatozoa: significance in the diagnosis and treatment of infertility. Minerva Ginecol. 2003, 55:233-9.

9. Alvarez JG . Nurture vs nature: how can we optimize sperm quality? J Androl. 2003, 24:640-8.

10. Fernandez JL, Muriel L, Vazquez R, Goyanes V, Alvarez JG . The Sperm Chromatin Decondensation Test: a Simple Method for the Determination of Sperm DNA Fragmentation. J Androl 2003; 24:59-66.

11. Saleh RA, Agarwal A, Nelson DR, Nada EA, El-Tonsy MH, Alvarez JG , Thomas AJ Jr, Sharma RK. Increased sperm nuclear DNA damage in normozoospermic infertile men: a prospective study. Fertil Steril. 2002;78:313-8.

12. Saleh RA, Agarwal A, Kandirali E, Sharma RK, Thomas AJ, Nada EA, Evenson DP, Alvarez JG . Leukocytospermia is associated with increased reactive oxygen species production by human spermatozoa. Fertil Steril. 2002;78:1215-24.

13. Alvarez JG , Ollero M. Characterization of human sperm. Hum Reprod. 2002;17:843.

14. Freedman SD, Weinstein D, Blanco PG, Martinez-Clark P, Urman S, Zaman M, Morrow JD, Alvarez JG . Characterization of LPS-induced lung inflammation in cftr(-/-) mice and the effect of docosahexaenoic acid. J Appl Physiol. 2002; 92:2169-76.

15. Saleh RA, Agarwal A, Kandirali E, Sharma RK, Thomas A, Nada EA, Evenson DP and Alvarez JG . Leukocytospermia is associated with increased leukocyte-induced reactive oxygen species production by human sperm. Fertil Steril. 2002;78:1215-24.

16. Alvarez JG , Sharma RK, Ollero M, Lopez MC, Saleh RA, Thomas A, Evenson DP and Agarwal A. Increased DNA damage in sperm from leukocytospermic semen samples as determined by the sperm chromatin structure assay. Fertil Steril 2002; 78:319-29.

17. Ollero M, Guzman-Gil E, Lopez MC, Sharma RK, Agarwal A, Larson K, Evenson DK, Thomas Jr. A, and Alvarez JG . Characterization of subsets of human spermatozoa at different stages of maturation: implications in the diagnosis and treatment of male infertility. Hum Reprod, 2001; 16:1912-21.

18. Gil-Guzman E, Ollero M, Lopez MC, Sharma RK, Agarwal A, and Alvarez JG . Differential production of reactive oxygen species by subsets of human spermatozoa at different stages of maturation. Hum Reprod. 2001; 16:1922-30.

19. Freedman SD, Weinstein D, Blanco P, Martinez-Clark P, Urman S, Zaman M, and Alvarez JG . Docosahexaenoic acid suppresses the enhanced cytokine and neutrophil response to aerosolized Pseudomonas endotoxin in lungs from cftr-/- mice. J Clin Inv, 2001 Methods Mol Med. 2002; 70:517-24.

20. Freedman SD , Shea JC, Blanco PG, Alvarez JG . Fatty acids in cystic fibrosis. Curr Opin Pulm Med 2000; 6:530-2.        

21. Ollero, M, Powers, DP, and Alvarez, JG . Variation of docosahexaenoic acid content in subsets of human spermatozoa at different stages of maturation: implications for sperm lipoperoxidative damage. Mol Reprod Dev, 2000; 55:326-334.

22. Freedman SD , Katz, MH, Parker E, Urman M, and Alvarez, JG . A membrane lipid imbalance plays a role in the phenotypic expression of cystic fibrosis in cftr -/- mice. Proc Natl Acad Sci 1999; 96:13995-14000.

23. Visconti PE , Galantino-Homer H, Ning XP, Moore GD, Valenzuela JP, Carolina J. Jorgez, Alvarez JG , and Kopf, GS. Cholesterol Efflux-Mediated Signal Transduction in Mammalian Sperm. J Biol Chem 1999; 29:3235:42.

24. Oshreoff, JE, Visconti PE, Ning XP, Valenzuela, JP, Travis, AJ, , Alvarez JG , and Kopf, GS. Regulation of Human Sperm Capacitation Efflux-Stimulated Signal Transduction Pathway Leading to Protein Kinase A-mediated Up-Regulation of Protein Tyrosine Phosphorylation. Mol Reprod Dev 1999; 5:1017-1026 .

25. Alvarez JG , Minaretzis D, Barrett CB, Mortola JF, Thompson I. The Sperm stress test: a novel test that predicts pregnancy in assisted reproductive technologies. Fertil Steril, 65:400-5, 1996.

26. Alvarez JG and Storey BT. Differential incorporation of fatty acids into and peroxidative loss of fatty acids from phospholipids of human spermatozoa. Mol Reprod Dev, 42:334-46,  1995.

27. Lasso JL, Noiles EE, Alvarez JG , and Storey BT. Mechanism of superoxide dismutase loss from human sperm cells during cryopreservation. J Androl, 15:255-65, 1994.

28. Alvarez JG , and Storey BT. Evidence that membrane stress contributes more than lipid peroxidation to sublethal cryodamage in cryopreserved human sperm: glycerol and other polyols as sole cryoprotectant. J Androl, 14:199-209, 1993.

29. Matsumura JE, George HJ, Ramachandran N, Alvarez JG , Strauss JF, and Billheimer JT. Expression of the mature and the pro-form of human sterol carrier protein 2 in Escherichia coli alters bacterial lipids.  Biochemistry, 32:567-572, 1993.

30. Alvarez JG , and Storey BT. Evidence for Increased Lipid Peroxidative damage resulting from freeze/thaw-induced loss of superoxide dismutase activity as a mode of sublethal cryodamage to human sperm during cryopreservation. J Androl, 13:232-41, 1992.

31. Alvarez JG , Storey BT, Hemling ML, and Grob RL. High-Resolution Proton nuclear magnetic resonance characterization of seminolipid from bovine spermatozoa. J Lipid Res, 31:1073-82, 1990.

32. Alvarez JG and Storey BT. Role of Glutathione peroxidase in protecting mammalian spermatozoa from loss of motility caused by spontaneous lipid peroxidation.  Gamete Res, 23:77-90, 1989.

33. Alvarez JG , Touchstone JC, Blasco, L, Storey BT. Spontaneous lipid peroxidation and production of hydrogen peroxide and superoxide in human spermatozoa.  Superoxide dismutase as major enzyme protectant against oxygen toxicity.  J Androl, 8:338-48, 1987.

34. Alvarez JG and Storey BT. Spontaneous lipid peroxidation in rabbit epididymal spermatozoa.  Biol. Reprod, 27:1102-08, 1982.

 Genética e infertilidad masculina

1. Egozcue J, Sarrate Z, Codina-Pascual M, Egozcue S , Oliver-Bonet M, Blanco J, Navarro J, Benet J, Vidal F. Meiotic abnormalities in infertile males. Cytogenet Genome Res. 2005;111:337-42. Review

2. Sarrate Z, Blanco J, Anton E, Egozcue S , Egozcue J, Vidal F. FISH studies of chromosome abnormalities in germ cells and its relevance in reproductive counseling. Asian J Androl. 2005; 7:227-36. Review.

3. Codina-Pascual,  Oliver-Bonet M, Navarro J, Campillo M, Garcia F , Egozcue S , Abad C, Egozcue J,  Benet J. Synapsis and meiotic recombination analyses: MLH1 focus in the XY pair as an indicator. Hum Reprod. 2005; 20:2133-9.

4. Sarrate Z, Blanco J, Egozcue S , Vidal F, Egozcue J. Identification of meiotic anomalies with multiplex fluorescence in situ hybridization: Preliminary results. Fertil Steril. 2004; 82:712-7.

5. Egozcue J, Blanco J, Anton E, Egozcue S , Sarrate Z, Vidal F. Genetic analysis of sperm and implications of severe male infertility--a review. Placenta. 2003; Suppl B:S62-5. Review.

6. Aran B, Vidal F, Vendrell JM, Garcia F , Egozcue S , Egozcue J, Barri PN, Veiga A. Outcome of intracytoplasmic sperm injection in relation to the meiotic pattern in patients with severe oligoasthenozoospermia. Fertil Steril. 2003; 80:91-5.

7. Vendrell JM, Aran B, Veiga A, Garcia F , Coroleu B, Egozcue S , Egozcue J, Barri PN. Spermatogenic patterns and early embryo development after intracytoplasmic sperm injection in severe oligoasthenozoospermia. J Assist Reprod Genet. 2003; 20:106-12.

8. Egozcue S , Blanco J, Vidal F. Egozcue J. Diploid sperm and the origin of triploidy. Hum Reprod. 2002; 17:5-7. Review.

9. Larriba S, Bassas L, Egozcue S , Gimenez J, Ramos MD, Briceno O, Estivill X, Casals T. Adenosine triphosphate-binding cassette superfamily transporter gene expression in severe male infertility. Biol Reprod. 2001; 65:394-400.

10. Egozcue S , Vendrell JM, Garcia F , Veiga A, Aran B, Barri PN, Egozcue J. Increased incidence of meiotic anomalies in oligoasthenozoospermic males preselected for intracytoplasmic sperm injection. J Assist Reprod Genet. 2000; 17:307-9.

11. Casals T, Bassas L, Egozcue S , Ramos MD; Gimenez J, Segura A, Garcia F , Carrera M, Larriba S, Sarquella J, Estivill X. Heterogeneity for mutations in the CFTR gene and clinical correlations in patients with congenital absence of the vas deferens. Hum Reprod. 2000; 15:1476-83.

12. Egozcue S , Blanco J, Vendrell JM, Garcia F , Veiga A, Aran B, Barri PN, Vidal F, Egozcue J. Human male infertility: chromosome anomalies, meiotic disorders, abnormal spermatozoa and recurrent abortion. Hum Reprod Update. 2000; 6:93-105. Review.

13. Aran B, Blanco J, Vidal F, Vendrell JM, Egozcue S , Barri PN, Egozcue J, Veiga A. Screening for abnormalities of chromosomes X, Y, and 18 and for diploidy in spermatozoa from infertile men participating in an in vitro fertilization-intracytoplasmic sperm injection program. Fertil Steril. 1999; 72:696-701.

14. Vendrell JM, Garcia F , Veiga A, Calderon G, Egozcue S , Egozcue J, Barri PN. Meiotic abnormalities and spermatogenic parameters in severe oligoasthenozoospermia. Hum Reprod. 1999 Feb;14:375-8.

Diagnóstico genético preimplantacional

1. Munne S, Velilla E , Colls P, Bermúdez MG , Vemuri MC, Steuerwald N, Garrisi J, Cohen J. Self-correction of chromosomally abnormal embryos in culture and implications for stem cell production. Fertil Steril. 2005; 84:1328-34.

2. Munne S, Chen S, Fischer J, Colls P, Zheng X, Stevens J, Escudero T, Oter M , Schoolcraft B, Simpson JL, Cohen J. Preimplantation genetic diagnosis reduces pregnancy loss in women aged 35 years and older with a history of recurrent miscarriages. Fertil Steril, 84:331-5, 2005.

3. Wells D, Bermudez MG , Steuerwald N, Malter HE, Thornhill AR, Cohen J. Association of abnormal morphology and altered gene expression in human preimplantation embryos. Fertil Steril, 84:343-55, 2005.

4. Wells D, Bermudez MG , Steuerwald N, Thornhill AR, Walker DL, Malter H, Delhanty JD, Cohen J. Expression of genes regulating chromosome segregation, the cell cycle and apoptosis during human preimplantation development. Hum Reprod., 20:1339-48, 2005.

5. Munne S, Velilla E , Colls P, Bermudez MG , Vemuri MC, Steuerwald N, Garrisi J, Cohen J. Self-correction of chromosomally abnormal embryos in culture and implications for stem cell production. Fertil Steril. 2005 Nov;84:1328-34, 2005.
6. Gutiérrez-Mateo C, Wells D, Benet J, Sánchez-García JF, Bermúdez MG , Belil I, Egozcue J, Munné S, Navarro J. Reliability of comparative genomic hybridization to detect chromosome abnormalities in first polar bodies and metaphase II oocytes . Hum. Reprod., 2004; 19: 2118 - 2125.

7. Munne S, Escudero T, Colls P, Xuezhong Z, Oter M , Garrisi M, Barnes F, Zouves C, Werlin L, Magli C, Cohen J. Predictability of preimplantation genetic diagnosis of aneuploidy and translocations on prospective attempts. Reprod Biomed Online, 9:645-51, 2004.

8. Gutiérrez-Mateo C, Benet J, Wells D, Colls P, Bermúdez MG , Sánchez-García JF, Egozcue J. Navarro J, Munné S. Aneuploidy study of human oocytes first polar body comparative genomic hybridization and metaphase II fluorescence in situ hybridization analysis. Hum. Reprod., 2004; 19: 2859 - 2868.

9. Munne S, Bahce M, Sandalinas M, Escudero T, Marquez C, Velilla E , Colls P, Oter M , Alikani M, Cohen J. Differences in chromosome susceptibility to aneuploidy and survival to first trimester. Reprod Biomed Online. 2004; 8:81-90.

10. Gutierrez-Mateo C, Benet J, Wells D, Colls P, Bermudez MG , Sanchez-Garcia JF, Egozcue J, Navarro J, Munne S. Aneuploidy study of human oocytes first polar body comparative genomic hybridization and metaphase II fluorescence in situ hybridization analysis. Hum Reprod, 19:2859-68, 2004.

11. Munne S, Bahce M, Sandalinas M, Escudero T, Marquez C, Velilla E , Colls P, Oter M , Alikani M, Cohen J. Differences in chromosome susceptibility to aneuploidy and survival to first trimester. Reprod Biomed Online, 8:81-90, 2004.

12. Bermudez MG , Wells D, Malter H, Munne S, Cohen J, Steuerwald NM. Expression profiles of individual human oocytes using microarray technology. Reprod Biomed Online, 8:325-37, 2004.

13. Gutierrez-Mateo C, Wells D, Benet J, Sanchez-Garcia JF, Bermudez MG , Belil I, Egozcue J, Munne S, Navarro J. Reliability of comparative genomic hybridization to detect chromosome abnormalities in first polar bodies and metaphase II oocytes. Hum Reprod, 19:2118-25, 2004.

14. Munne S, Sandalinas M, Escudero T, Velilla E , Walmsley R. Sadowy S, Cohen J, Sable D. Improved implantation after preimplantation genetic diagnosis of aneuploidy. Reprod Biomed Online. 2003; 7:91-7.

15. Munne S, Sandalinas M, Escudero T, Velilla E , Walmsley R, Sadowy S, Cohen J, Sable D. Improved implantation after preimplantation genetic diagnosis of aneuploidy. Reprod Biomed Online, 7:91-7, 2003.

16. Bermudez MG , Piyamongkol W, Tomaz S, Dudman E, Sherlock JK, Wells D. Single-cell sequencing and mini-sequencing for preimplantation genetic diagnosis. Prenat Diagn, 23:669-77, 2003.

17. Piyamongkol W, Bermudez MG , Harper JC, Wells D. Detailed investigation of factors influencing amplification efficiency and allele drop-out in single cell PCR: implications for preimplantation genetic diagnosis. Mol Hum Reprod, 9:411-20, 2003.

18. Blanco J, Farreras A , Egozcue J, Vidal F. Meiotic Behavious of the sex chromosomes in a 45,X/46, Xr(Y)/46,X, dic r(Y) patient whose semen was assessed by fluorescence in situ hybridization. Fertil Steril, 79:913-8, 2003.

19. Velilla E , Escudero T, Munne S. Blastomere fixation techniques and risk of misdiagnosis for preimplantation genetic diagnosis of aneuploidy. Reprod Biomed Online. 2002; 4:210-7.

20. Velilla E , Escudero T, Munne S. Blastomere fixation techniques and risk of misdiagnosis for preimplantation genetic diagnosis of aneuploidy. Reprod Biomed Online, 4:210-7, 2002.

21. Lavery SA, Aurell R, Turner C, Castello C , Veiga A, Barri PN, Winston RM. Preimplantation genetic diagnosis: patients´experiences and attitudes. Hum Reprod, 17:2464-7, 2002.

22. Gutierrez-Adan A, Oter M , Martinez-Madrid B, Pintado B, De La Fuente J. Differential expression of two genes located on the X chromosome between male and female in vitro-produced bovine embryos at the blastocyst stage. Mol Reprod Dev, 55:146-51, 2000.